abstract：:6-Oxopiperidine-2-carboxylic acid (OCA; cyclic alpha-aminoadipic acid) reverses the L-lysine inhibition of penicillin G production by Penicillium chrysogenum PQ-96. The reaction probably depends on the recovery of L-alpha-aminoadipic acid for penicillin G production from OCA. ...

journal_title：Applied microbiology and biotechnology

authors： Kurzatkowski W,Kurzatkowski JD,Filipek J,Solecka J,Kuryłowicz W

更新日期：1990-12-01 00:00:00

abstract：:Recombinant DNA technology was used to insert a fetal liver genomic library ApaI fragment encoding for human erythropoietin (Epo) into Bowes melanoma cells. The cells expressed the erythropoietin gene, and Epo was secreted into the culture medium together with the normally-secreted tissue plasminogen activator. Attemp...

journal_title：Applied microbiology and biotechnology

authors： Keay L,Walsh JM,Buchholz RM,Terando JM

更新日期：1990-11-01 00:00:00

abstract：:For continuous production of the antibiotic nikkomycin immobilized cells have been used in a fluidized bed bioreactor. Cells of Streptomyces tendae were immobilized on sintered glass particles. Different biomass concentrations on the particles correspond to different thicknesses of mycelial layers because growth occur...

journal_title：Applied microbiology and biotechnology

authors： Trück HU,Chmiel H,Hammes WP,Trösch W

更新日期：1990-10-01 00:00:00

abstract：:The production of human gamma interferon as intracellular inclusion bodies in Escherichia coli, which simplified the purification process, is described. An expression plasmid carrying lipoprotein and the tryptophane promoters in tandem was used. Preparation of highly pure interferon was achieved using high resolution ...

journal_title：Applied microbiology and biotechnology

authors： Perez L,Vega J,Chuay C,Menendez A,Ubieta R,Montero M,Padron G,Silva A,Santizo C,Besada V

更新日期：1990-07-01 00:00:00

abstract：:Biofilm development in methanogenic fluidized-bed reactors with sand as the carrier was studied on a laboratory scale. The microorganisms present in consecutive layers of the biofilm of mature sludge granules were preliminarily characterized on the basis of their morphology, element composition and adhesion capacity a...

journal_title：Applied microbiology and biotechnology

authors： Lauwers AM,Heinen W,Gorris LG,van der Drift C

更新日期：1990-06-01 00:00:00

abstract：:Influence of pressure (P) and oxygen partial pressure (PO2) on cultivation of various Streptomyces spp. and Micromonospora purpurea was examined in pressurized air-lift and stirred tank fermenters. The maximum PO2 was 2100 mbar. Growth and product formation of all cultures tested were markedly influenced by PO2 higher...

journal_title：Applied microbiology and biotechnology

authors： Liefke E,Kaiser D,Onken U

更新日期：1990-03-01 00:00:00

abstract：:We have characterized a mutant of Streptomyces clavuligerus NRRL 3585 which is almost completely blocked in cephalosporin biosynthesis and exhibits depressed activities of both the delta(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) synthetase and cyclase enzymes of the cephalosporin pathway. A wild-type DNA region ...

journal_title：Applied microbiology and biotechnology

authors： Piret J,Resendiz B,Mahro B,Zhang JY,Serpe E,Romero J,Connors N,Demain AL

更新日期：1990-02-01 00:00:00

abstract：:Streptomyces tendae Tü 901 produces the nucleoside peptide antibiotic nikkomycin. In shot-gun cloning experiments using pIJ699 as vector we isolated a 9.4-kb DNA fragment from S. tendae which complemented the nikkomycin nonproducing mutant NP9 to the formation of nikkomycin C/Cx and Kx. Nikkomycins were identified by ...

journal_title：Applied microbiology and biotechnology

authors： Bormann C,Aberle K,Fiedler HP,Schrempf H

更新日期：1990-01-01 00:00:00